Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.

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Gibco™ MEM α, no nucleosides, powder

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Form	Powder
Without Additives	No HEPES,No Ribonucleosides,No Deoxyribonucleosides,No Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type	MEM α (Minimum Essential Medium α)
Classification	Animal Origin-free
Shelf Life	36 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ AmnioMAX™ C-100 Basal Medium

Part of kit used to prepare fully-supplemented Gibco AmnioMAX C-100 Complete Medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Form	Liquid
Sterility	Sterile-filtered

Gibco™ MEM

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
Sterilization Method	Sterile-filtered
With Additives	Low Glucose,Glutamine,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ DMEM, low glucose, GlutaMAX™ Supplement, pyruvate

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	GlutaMAX,Low Glucose,Sodium Pyruvate,Phenol Red
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1X
Sterility	Sterile-filtered

Gibco™ Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Reduced Serum

Gibco™ Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Cell Line	Sp2, Vero, Raji, Daudi, and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Reduced Serum

Gibco™ Fischer's Medium

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells.

Pricing & Availability

Gibco™ RPMI 1640 Medium, powder

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability

Gibco™ FreeStyle™ F17 Expression Medium

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

Pricing & Availability
Specifications

Cell Line	HEK293
Culture Environment	CO₂
Purity or Quality Grade	Qualified for Further Manufacturing
Form	Liquid
Without Additives	No Glutamine
Endotoxin Level	< 1 EU/mL
Concentration	1 X
For Use With (Application)	Protein expression, viral vector production
Sterility	Sterile
Product Type	Expression Medium
Classification	Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type	Suspension Cell Culture
Product Line	Freestyle™
Serum Level	Serum-free

Gibco™ MEM, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Form	Powder
Without Additives	No HEPES,No Sodium Pyruvate,No Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,Glutamine,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	36 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ MEM, HEPES, GlutaMAX™ Supplement

Minimum Essential Medium is commonly used of all cell culture media

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Sodium Pyruvate
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,GlutaMAX,HEPES,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™, GlutaMAX™
Serum Level	Standard Serum Supplementation

Gibco™ MEM α, nucleosides, no phenol red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Phenol Red
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
Sterilization Method	Sterile-filtered
With Additives	Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type	MEM α (Minimum Essential Medium α)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Sp2, AE-1, CHO, BHK-21, and HEK
Content And Storage	Store at 2–8°C and protect from light.
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts
Sterilization Method	Sterile-filtered
With Additives	Glutamine,Phenol Red
Product Type	Opti-MEM I
Classification	Human Origin
Shelf Life	18 Months From Date of Manufacture
Product Line	Gibco™, Opti-MEM™

Classical and Basal Cell Culture Media

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Gibco™ AmnioMAX™ C-100 Basal Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, GlutaMAX™ supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, low glucose, GlutaMAX™ Supplement, pyruvate Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced RPMI 1640 Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Fischer's Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ FreeStyle™ F17 Expression Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, HEPES, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Opti-MEM™ I Reduced Serum Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More